Dr. Johannes Gebert
As a molecular biologist by training, my scientific interests are driven by understanding basic mechanisms of tumor biology. Apart from diagnostic screening approaches, most of my previous and current work focussed on tumor-specific alterations of specific receptors and their disease-specific consequences. I have gained a broad practical and theoretical expertise in the molecular and cellular biology of human tumors. My more recent research has been directed towards the molecular mechanisms of MSI tumorigenesis in MMR-deficient mice. As a PI and Co-Investigator my research has been secured through funding by several funding agencies. Major efforts of my future research are devoted to the altered biology of human and mouse MSI tumor cells and the therapeutic concepts derived thereof (https://scholar.google.de/citations?hl=de&user=xhRRgqcAAAAJ).
Publications (selected) / Output
Katzenmaier EM, Kloor M, Gabius HJ, Gebert J, Kopitz J. Analyzing epigenetic control of galectin expression indicates silencing of galectin-12 by promoter methylation in colorectal cancer. IUBMB Life. 2017 Dec;69(12):962-970. PMID: 29098769.
Fricke F, Lee J, Michalak M, Warnken U, Hausser I, Suarez-Carmona M, Halama N, Schnölzer M, Kopitz J, Gebert J. TGFBR2-dependent alterations of exosomal cargo and functions in DNA mismatch repair-deficient HCT116 colorectal cancer cells. Cell Commun Signal. 2017 Apr 4;15(1):14. PMID: 28376875
Gebert J, Schnölzer M, Warnken U, Kopitz Combining Click Chemistry-Based Proteomics With Dox-Inducible Gene Expression. Methods Enzymol. 2017;585:295-327. PMID: 28109436
Lee J, Katzenmaier EM, KopitzJ, Gebert J. Reconstitution of TGFBR2 in HCT116 colorectal cancer cells causes increased LFNG expression and enhanced N-acetyl-d-glucosamine incorporation into Notch1. Cell Signal. 2016 Aug;28(8):1105-13. doi: 10.1016/j.cellsig.2016.04.012. Epub 2016 May 5. PMID: 27156840
LeeJ, Fricke F, Warnken U, Schnölzer M, Kopitz J, Gebert Reconstitution of TGFBR2-Mediated Signaling Causes Upregulation of GDF-15 in HCT116 Colorectal Cancer Cells. PLoS One. 2015 Jun 26;10(6):e0131506. PMID: 26114631
Woerner SM, Tosti E, Yuan YP, Kloor M, Bork P, Edelmann W, Gebert J. Detection of coding microsatellite frameshift mutations in DNA mismatch repair-deficient mouse intestinal tumors. Mol Carcinog. 2015 Nov;54(11):1376-86. PMID: 25213383
Lee J, Warnken U, Schnölzer M, Gebert J, Kopitz A new method for detection of tumor driver-dependent changes of protein sialylation in a colon cancer cell line reveals nectin-3 as TGFBR2 target. Protein Sci. 2015 Oct;24(10):1686-94. PMID: 26177744
Ballikaya S, Lee J, Warnken U, Schnölzer M, Gebert J, Kopitz De Novo proteome analysis of genetically modified tumor cells by a metabolic labeling/azide-alkyne cycloaddition approach. Mol Cell Proteomics. 2014 Dec;13(12):3446-56. Epub 2014 Sep 15. PMID: 25225355
Lee J, Ballikaya S, Schönig K, Ball CR, Glimm H, Kopitz J, Gebert J. Transforming growth factor beta receptor 2 (TGFBR2) changes sialylation in the microsatellite unstable (MSI) Colorectal cancer cell line HCT116. PLoS One. 2013;8(2):e57074. PMID: 23468914
Gebert J, Kloor M, Lee J, Lohr M, André S, Wagner R, Kopitz J, Gabius HJ. Colonic carcinogenesis along different genetic routes: glycophenotyping of tumor cases separated by microsatellite instability/stability. Histochem Cell Biol. 2012 Aug;138(2):339-50. PMID: 22565205
Roeckel N, Woerner SM, Kloor M, Yuan YP, Patsos G, Gromes R, Kopitz J, Gebert J. High frequency of LMAN1 abnormalities in colorectal tumors with microsatellite instability. Cancer Res. 2009 Jan 1;69(1):292-9. PMID:19118014
Korff S, Woerner SM, Yuan YP, Bork P, von Knebel Doeberitz M, Gebert J. Frameshift mutations in coding repeats of protein tyrosine phosphatase genes in colorectal tumors with microsatellite instability. BMC Cancer. 2008 Nov 10;8:329. PMID: 19000305
Woerner SM, Kloor M, von Knebel Doeberitz M, Gebert JF. Microsatellite instability in the development of DNA mismatch repair deficient tumors.Cancer Biomark. 2006;2(1-2):69-86. Review. PMID: 17192061
André S, Kaltner H, Lensch M, Russwurm R, Siebert HC, Fallsehr C, Tajkhorshid E, Heck AJ, von Knebel Doeberitz M, Gabius HJ, Kopitz Determination of structural and functional overlap/divergence of five proto-type galectins by analysis of the growth-regulatory interaction with ganglioside GM1 in silico and in vitro on human neuroblastoma cells. Int J Cancer. 2005 Mar 10;114(1):46-57. PMID: 15523676